Simple Instructions for making 0.2M Phosphate Buffer
Mar 5, 01:05 PM
Make up two separate solutions. One of Sodium Di-Hydrogen Orthophosphate (NaH2PO4) and one of Di-Sodium Hydrogen Phosphate (Na2HPO4). Make up each to a concentration of 0.2M.
For 0.2M NaH2PO4:
Check the molecular weight of the “kind” of NaH2PO4 you are using.
For NaH2PO4H2O: Mol. Weight = 137.99.
∴ 0.2M = 27.598g/L
For 0.2M Na2HPO4:
Check the molecular weight of the “kind” of Na2HPO4 you are using.
For anhydrous Na2HPO4: Mol. Weight = 141.96.
∴ 0.2M = 28.392g/L
Mix each solution in the following proportions to achieve the desired pH:
| NaH2PO4 | Na2HPO4 | pH |
|---|---|---|
| .975 | .025 | 5.3 |
| .950 | .050 | 5.6 |
| .900 | .100 | 5.91 |
| .800 | .200 | 6.24 |
| .700 | .300 | 6.47 |
| .600 | .400 | 6.64 |
| .500 | .500 | 6.81 |
| .400 | .600 | 6.98 |
| .300 | .700 | 7.17 |
| .200 | .800 | 7.38 |
| .100 | .900 | 7.73 |
| .050 | .950 | 8.04 |
Thanks to Vickey, who gave me this method. Where she got it from I have no idea.
